Insertion junction: LMJ.RY0402.039689_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.039689
Chromosome:chromosome 3
Location:8351283
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g198800 (1 of 1) PTHR24078:SF169 - DNAJ HOMOLOG SUBFAMILY C MEMBER 17 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTTTCCGGCGCTGACCGCGCAGGTTCTG
Internal bar code:TTCGGGGGATACAGTAACGCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:510
LEAP-Seq percent confirming:99.7496
LEAP-Seq n confirming:1195
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:AAACACATGCGAAAAGACCC
Suggested primer 2:AGCCATACCTGTTGTGAGGG