| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039691 |
| Chromosome: | chromosome 13 |
| Location: | 2314958 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g579100 | APC1 | (1 of 1) K03348 - anaphase-promoting complex subunit 1 (APC1); Anaphase promoting complex subunit 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCCGCCTGTGCCCCCCCTCTTCTCCACT |
| Internal bar code: | CGCACAGCCGAGTGACGGTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 210 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGTGAACTGCCGTAATGG |
| Suggested primer 2: | CGTTGGTAATGGCTTCGTTT |