Insertion junction: LMJ.RY0402.039692_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.039692
Chromosome:chromosome 6
Location:1282972
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g258226 (1 of 5) 3.2.1.23 - Beta-galactosidase / Lactase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAACCCACTACCCCACACTCACCACGCA
Internal bar code:GTGACATTTCATGCGAGGGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:523
LEAP-Seq percent confirming:79.8897
LEAP-Seq n confirming:2463
LEAP-Seq n nonconfirming:620
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ACAAGGGACGAAACACGAAC
Suggested primer 2:TGCTCCTCCTACTCCTTCCA