Insertion junction: LMJ.RY0402.039695_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.039695
Chromosome:chromosome 7
Location:3949482
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g339450 (1 of 12) IPR004843//IPR029052 - Calcineurin-like phosphoesterase domain, apaH type // Metallo-dependent phosphatase-like 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACATCCCCACGGGCCACGGACTGCGCAGCG
Internal bar code:ACTTGATATTAGAACGGGACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:607
LEAP-Seq percent confirming:98.2383
LEAP-Seq n confirming:2342
LEAP-Seq n nonconfirming:42
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCGTGTACAGTTGCTAGA
Suggested primer 2:GTAGCAGTAGCAACCCCAGC