| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039746 |
| Chromosome: | chromosome 2 |
| Location: | 1079137 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g080900 | PRX4 | (1 of 1) PTHR10430//PTHR10430:SF19 - PEROXIREDOXIN // SUBFAMILY NOT NAMED; Peroxiredoxin, type II | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTCGATTGGCCTTCGGGGCGTTGGCTCA |
| Internal bar code: | CGTCTTTAAGCGAGTGTATGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 134 |
| LEAP-Seq percent confirming: | 61.5385 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTTGCGCTAACCAAGGAG |
| Suggested primer 2: | GATCCCCCAGTCTATCCCAT |