| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.039749 |
| Chromosome: | chromosome_10 |
| Location: | 878967 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g424000 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGGGCTCAGTGGACCCCTACGCCCAGGTGC |
| Internal bar code: | GAAGATTCTGCCAATACCCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 560 |
| LEAP-Seq percent confirming: | 13.0467 |
| LEAP-Seq n confirming: | 1220 |
| LEAP-Seq n nonconfirming: | 8131 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGAGTCCGAGTACCAAGC |
| Suggested primer 2: | TATCGTGCCAGCTGCAATAG |