Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.039749 |
Chromosome: | chromosome_10 |
Location: | 878969 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g424000 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCTCCCATCATCATGCCTCCCGGAGAGCCC |
Internal bar code: | TTAGCTCCGCTTGAAGGCCGAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 156 |
LEAP-Seq percent confirming: | 56.7568 |
LEAP-Seq n confirming: | 21 |
LEAP-Seq n nonconfirming: | 16 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCGAGTCCGAGTACCAAGC |
Suggested primer 2: | TATCGTGCCAGCTGCAATAG |