| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.039762 |
| Chromosome: | chromosome_6 |
| Location: | 2173578 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g265950 | DHC3 | Flagellar inner arm dynein heavy chain, novel minor type dynein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATCCTTCACAGTGAGCCGCAAAGAACACGT |
| Internal bar code: | AGATCCTGGTGTGCCATCGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 778 |
| LEAP-Seq percent confirming: | 99.4814 |
| LEAP-Seq n confirming: | 6906 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGCGTGTTCTGATGTGTA |
| Suggested primer 2: | GCGCTGCATTTACTTTGTGA |