| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039772 |
| Chromosome: | chromosome_8 |
| Location: | 1737203 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g366101 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAGGTGGGAGCAGTGGAGCAAAACAGGGGT |
| Internal bar code: | GTTGACGTGCCCATCAACTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 651 |
| LEAP-Seq percent confirming: | 98.6851 |
| LEAP-Seq n confirming: | 9231 |
| LEAP-Seq n nonconfirming: | 123 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGCACCCTATCAACACCA |
| Suggested primer 2: | TTTTGGAGGAGGGTATGTCG |