Insertion junction: LMJ.RY0402.039772_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.039772
Chromosome:chromosome 8
Location:1737209
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g366101 (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGCTCAGGACGGGCGGACGCCGCTGTACG
Internal bar code:GTGGGATACCAAGCGAATGCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:290
LEAP-Seq percent confirming:98.9995
LEAP-Seq n confirming:5838
LEAP-Seq n nonconfirming:59
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:TCAGCACCCTATCAACACCA
Suggested primer 2:TTTTGGAGGAGGGTATGTCG