| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.039781 |
| Chromosome: | chromosome 4 |
| Location: | 3116169 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g225800 | CPLD71 | dihydrolipoyllysine-residue acetyltransferase; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCATCATGCCGTCACCGCCTCCTCCATCA |
| Internal bar code: | GGACGTGCCGTCCCCGGTAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 631 |
| LEAP-Seq percent confirming: | 95.5513 |
| LEAP-Seq n confirming: | 494 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGGCATACTGGTCCCTAA |
| Suggested primer 2: | AACAGCTGCAGAGGGAGAAA |