| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.039814 |
| Chromosome: | chromosome 7 |
| Location: | 4633268 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344260 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTCTTGATATGCGGCTGGGCCACGCCG |
| Internal bar code: | AGCGGGGTGTGCCACAGTTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 130 |
| LEAP-Seq percent confirming: | 97.2028 |
| LEAP-Seq n confirming: | 139 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAATTCGCCGAAATTGACG |
| Suggested primer 2: | TAAGCAGGAGTGTGTCGTGC |