| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039824 |
| Chromosome: | chromosome 2 |
| Location: | 2742452 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g093850 | (1 of 1) IPR000104//IPR000595//IPR001611//IPR003591//IPR018490 - Antifreeze protein, type I // Cyclic nucleotide-binding domain // Leucine-rich repeat // Leucine-rich repeat, typical subtype // Cyclic nucleotide-binding-like | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGAAGCTGGTCTCGCGCCCCTGCAGGGT |
| Internal bar code: | TGAGGGGTGTGTGACCGCCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1274 |
| LEAP-Seq percent confirming: | 99.4984 |
| LEAP-Seq n confirming: | 5356 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACAAAGGAGTCGGGTTGG |
| Suggested primer 2: | CCCTCTGTAACATCCGCATT |