| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039846 |
| Chromosome: | chromosome 16 |
| Location: | 334521 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g694208 | (1 of 1) IPR001965//IPR011011//IPR019787//IPR028941 - Zinc finger, PHD-type // Zinc finger, FYVE/PHD-type // Zinc finger, PHD-finger // WHIM2 domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTCAGACCAGCAATCCAGCGCCCCCGGC |
| Internal bar code: | AGCCCTCTAGAGTTCGTTGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 353 |
| LEAP-Seq percent confirming: | 98.2521 |
| LEAP-Seq n confirming: | 3710 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTATGACCCGTCGCTCATT |
| Suggested primer 2: | TCTGCATCTGCGTGTTAAGG |