| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.039865 |
| Chromosome: | chromosome 17 |
| Location: | 2775299 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g718600 | (1 of 4) 3.1.1.23 - Acylglycerol lipase / Monoacylglycerol lipase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAGTGAAGCTGCCCATGCGTCTGCCGCC |
| Internal bar code: | GTGATTGGGCAGTAGAGCGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 464 |
| LEAP-Seq percent confirming: | 99.6853 |
| LEAP-Seq n confirming: | 8553 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTCCACAACCACTATCGC |
| Suggested primer 2: | GACATGTTGTGGTGAGCAGG |