| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039886 |
| Chromosome: | chromosome_5 |
| Location: | 1186795 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g245500 | FAP175,ANK24 | Flagellar Associated Protein with ankyrin repeats | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCGCACGCGAGGTGCAGCGCCGTTTTGCCG |
| Internal bar code: | TCTAGTTTAGTCTTAGAGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1227 |
| LEAP-Seq percent confirming: | 99.4104 |
| LEAP-Seq n confirming: | 8599 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGGATGTGAGTATGGTGA |
| Suggested primer 2: | ATCTGCCTACCTTGGTGGTG |