Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.039895 |
Chromosome: | chromosome 17 |
Location: | 2299783 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g714150 | KUP1 | (1 of 5) K03549 - KUP system potassium uptake protein (kup); Potassium ion uptake transporter | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGACTAGGTGATTGTTGAGAGGAGCTGA |
Internal bar code: | GGAATGTCGCAGCGGTAAGCAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 896 |
LEAP-Seq percent confirming: | 97.4718 |
LEAP-Seq n confirming: | 2159 |
LEAP-Seq n nonconfirming: | 56 |
LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAATGTCAGTGGTGGAGGTG |
Suggested primer 2: | CTGAAGAAGGGCAAGTACGC |