| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.039907 |
| Chromosome: | chromosome 3 |
| Location: | 5764304 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g188550 | HEL20 | (1 of 1) K14806 - ATP-dependent RNA helicase DDX31/DBP7 [EC:3.6.4.13] (DDX31, DBP7); DEAD/DEAH box helicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGATGTGTAAGAACAGCGTTGGGCGCGAA |
| Internal bar code: | CCGTATTTTACTTCGCAGGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 744 |
| LEAP-Seq percent confirming: | 98.3996 |
| LEAP-Seq n confirming: | 6333 |
| LEAP-Seq n nonconfirming: | 103 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGATTAAGGTTGGGTTTGG |
| Suggested primer 2: | GTGTGTGTGTCCCAAGATGC |