| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.039915 |
| Chromosome: | chromosome 1 |
| Location: | 906585 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004850 | TXE1 | Transcription elongation factor; (1 of 1) PTHR30195//PTHR30195:SF13 - TYPE I SITE-SPECIFIC DEOXYRIBONUCLEASE PROTEIN SUBUNIT M AND R // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGCAGGTAGGTGACGTCATAGAACTCC |
| Internal bar code: | GCTTATCATTCTGTACACTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 23 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCTAAGCTTGCGAGTCAA |
| Suggested primer 2: | CTGGCAGAAGCCAAAATAGC |