| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.039953 |
| Chromosome: | chromosome 2 |
| Location: | 3593244 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095137 | PFR1 | (1 of 1) 1.2.7.1 - Pyruvate synthase / Pyruvic-ferredoxin oxidoreductase; Pyruvate ferredoxin oxidoreductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAAGGCCTCCCGGCGGCGTGCATCCCAC |
| Internal bar code: | GCTGCACAAGGGGGTCGAGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 480 |
| LEAP-Seq percent confirming: | 99.7236 |
| LEAP-Seq n confirming: | 1443 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCACCAACATCACCAACG |
| Suggested primer 2: | ACGGACATGACGTTGTTGAA |