| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.039965 |
| Chromosome: | chromosome 1 |
| Location: | 6767236 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g048701 | (1 of 3) IPR002913 - START domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTCCTCTTAGGTTTGGCTGTGTTCATGG |
| Internal bar code: | TGGGGCTGTGGTTCACAACGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 452 |
| LEAP-Seq percent confirming: | 99.5761 |
| LEAP-Seq n confirming: | 9867 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCACACACACACACACAC |
| Suggested primer 2: | TGTGAGCGGTATCAGGAGTG |