| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039970 |
| Chromosome: | chromosome 2 |
| Location: | 1586954 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085000 | (1 of 1) PTHR22847//PTHR22847:SF423 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGTTGTGACCAGAGGTCGCTGGTCAGG |
| Internal bar code: | CTCGCAAGTAGGTTCAGCGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 646 |
| LEAP-Seq percent confirming: | 97.5799 |
| LEAP-Seq n confirming: | 7056 |
| LEAP-Seq n nonconfirming: | 175 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCGTACCCTCATACACCA |
| Suggested primer 2: | CGAGCTGGAGTTACGTGTGA |