Insertion junction: LMJ.RY0402.039975_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.039975
Chromosome:chromosome 12
Location:3753476
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g515050 HLM18,SET4 putative N-methyltransferase; (1 of 1) PTHR13793:SF5 - HISTONE-LYSINE N-METHYLTRANSFERASE ATX4-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGGGAGCGGGGTGGAGAGCGGAGACGTAC
Internal bar code:TCAGCTCATCTACATGTATTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:835
LEAP-Seq percent confirming:99.6442
LEAP-Seq n confirming:3641
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCCGCCACACATCATACA
Suggested primer 2:TCCTCAACACACAAACGACC