Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.039986 |
Chromosome: | chromosome 17 |
Location: | 5070766 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g734805 | (1 of 8) PF00560 - Leucine Rich Repeat (LRR_1) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACCCGCCGGCCGTGCCGAAACACAGCGA |
Internal bar code: | GTCATAGGGGTCTGATAATGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 690 |
LEAP-Seq percent confirming: | 93.2643 |
LEAP-Seq n confirming: | 180 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCACTTCACTGCTACTGCCA |
Suggested primer 2: | GTTAGTATCCCCGCCTGTCA |