| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.040007 |
| Chromosome: | chromosome 3 |
| Location: | 2654710 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g160953 | (1 of 1) 3.1.27.1//3.6.3.44 - Ribonuclease T(2) / Ribonuclease T2 // Xenobiotic-transporting ATPase / Steroid-transporting ATPase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGGGGCCGTAGGCGGCAGTCAGGGCAG |
| Internal bar code: | CTTCTGTTCGGAGCTGATGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 758 |
| LEAP-Seq percent confirming: | 91.5529 |
| LEAP-Seq n confirming: | 32721 |
| LEAP-Seq n nonconfirming: | 3019 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTCTCTCCTCACCTGCGT |
| Suggested primer 2: | GGGAGGCTGATGAGGTATGA |