| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.040019 |
| Chromosome: | chromosome_11 |
| Location: | 2533660 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g476050 | ODA2,DHC15,DYHG | Flagellar outer arm dynein heavy chain gamma | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GATAGAAGAGCGTGTCACCCATTCATTAGA |
| Internal bar code: | CCTCCTTGACTATGTACCTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 583 |
| LEAP-Seq percent confirming: | 99.4997 |
| LEAP-Seq n confirming: | 1790 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGGTGAGTTTGGGTTCCT |
| Suggested primer 2: | AGGAATTTTGATGGTGGCTG |