Insertion junction: LMJ.RY0402.040036_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.040036
Chromosome:chromosome 17
Location:1096676
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g703950 (1 of 2) 1.1.3.30 - Polyvinyl-alcohol oxidase / PVA oxidase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCCTAAAGTGCAGGCAGTACGGTCTAACT
Internal bar code:CGTACGCGACCGGGATGACGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:213
LEAP-Seq percent confirming:45.1649
LEAP-Seq n confirming:4012
LEAP-Seq n nonconfirming:4871
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCCGTGACGTTCTTCTTC
Suggested primer 2:CACCCTGGTCAATGTAACCC