| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.040036 |
| Chromosome: | chromosome 17 |
| Location: | 1096685 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g703950 | (1 of 2) 1.1.3.30 - Polyvinyl-alcohol oxidase / PVA oxidase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACCTTGCTCCAGGAATACCAATTTGTAC |
| Internal bar code: | CACTTAGTGCTTGACTAGTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 7.22952 |
| LEAP-Seq n confirming: | 286 |
| LEAP-Seq n nonconfirming: | 3670 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCCGTGACGTTCTTCTTC |
| Suggested primer 2: | CACCCTGGTCAATGTAACCC |