| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.040079 |
| Chromosome: | chromosome 2 |
| Location: | 1142783 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g081600 | PFH8,PHX3,P4H8 | Prolyl 4-hydroxylase 8; (1 of 14) K00472 - prolyl 4-hydroxylase (E1.14.11.2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCGAGGCGTGCGTGTCCTCCGTGTTGCC |
| Internal bar code: | TTCTAATTAACTTGATGTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 78 |
| LEAP-Seq percent confirming: | 94.8052 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAACCTGCCTTACCACTG |
| Suggested primer 2: | GACCAGTGTGCCGTAATCCT |