| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.040124 |
| Chromosome: | chromosome 13 |
| Location: | 1845336 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575300 | (1 of 1) K08876 - SCY1-like protein 1 (SCYL1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCCCATCGGGGGTACAAGCACGCATGC |
| Internal bar code: | AGCGGGAGTAAGGTCTGTCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 953 |
| LEAP-Seq percent confirming: | 98.8586 |
| LEAP-Seq n confirming: | 1559 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCGTCTTGCTAATGTGTA |
| Suggested primer 2: | TCAGCTTCACATTTTCGTCG |