| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.040141 |
| Chromosome: | chromosome 10 |
| Location: | 4259438 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450500 | (1 of 4) 2.7.9.5 - Phosphoglucan, water dikinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGGGATGTTCGCGTGGGTGACGGAGAAG |
| Internal bar code: | ATCTAGGATGCGTGGCGCCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 631 |
| LEAP-Seq percent confirming: | 98.9141 |
| LEAP-Seq n confirming: | 5283 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATTACCAGGGAGTGCAACG |
| Suggested primer 2: | CATCGCTTGATCGGGTTACT |