Insertion junction: LMJ.RY0402.040145_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.040145
Chromosome:chromosome 3
Location:7270015
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g207351 (1 of 2) PF10998 - Protein of unknown function (DUF2838) (DUF2838) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTAGCTGCCGCCGTGCCAGATGGACGCAC
Internal bar code:CATCGTGCCCGGACCGATCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:911
LEAP-Seq percent confirming:96.8958
LEAP-Seq n confirming:7585
LEAP-Seq n nonconfirming:243
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGGTTGCAGACGCTGATA
Suggested primer 2:CGCGTGTGCGTAACTTCTAA