| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.040153 |
| Chromosome: | chromosome 5 |
| Location: | 819038 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g248050 | (1 of 1) IPR012889 - L-fucose isomerase, N-terminal-2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTGCCTCAGGCGAGCCAGCTCGCCCTG |
| Internal bar code: | TTATCGCGCCGGGCCCGTACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 150 |
| LEAP-Seq percent confirming: | 99.5422 |
| LEAP-Seq n confirming: | 3914 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCAGCCCAAACTAAACCG |
| Suggested primer 2: | CTGCTGGGAGATTTTGCTCT |