| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.040162 |
| Chromosome: | chromosome 10 |
| Location: | 3687003 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g445952 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGTGGGAAGGCGCTCTTGAGCTGGTCT |
| Internal bar code: | GTGTTTCCTGTATGATGTGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 692 |
| LEAP-Seq percent confirming: | 99.547 |
| LEAP-Seq n confirming: | 12087 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGGTAGACGGACAGCACC |
| Suggested primer 2: | TCTCAAGTGCCACAGCAGTC |