| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.040174 |
| Chromosome: | chromosome 13 |
| Location: | 2207741 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578451 | TSL1 | (1 of 2) 6.1.1.4 - Leucine--tRNA ligase / Leucyl-tRNA synthetase; Leucyl-tRNA synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTGAGGTTGTTTGTGTCGGGTGGAGGG |
| Internal bar code: | GATCACTGCCACGACCGTACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 241 |
| LEAP-Seq percent confirming: | 99.6753 |
| LEAP-Seq n confirming: | 5218 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCATGTCCTTGATGCTGT |
| Suggested primer 2: | GAGAGATCTCCACCACCGAC |