| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.040181 |
| Chromosome: | chromosome 5 |
| Location: | 2227254 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234657 | (1 of 1) 2.7.7.87 - L-threonylcarbamoyladenylate synthase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCTTTCAAGGGCGGCGGCGGGGGGGGGC |
| Internal bar code: | GGTCATAGCGTTCACCCCCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 550 |
| LEAP-Seq percent confirming: | 98.6072 |
| LEAP-Seq n confirming: | 4885 |
| LEAP-Seq n nonconfirming: | 69 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCGTCCGTCTTTCAATC |
| Suggested primer 2: | GCTGTCAATGCTCAAGTCCA |