Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.040275 |
Chromosome: | chromosome_17 |
Location: | 2157398 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g712900 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACAAATACACACACGCCACTTACCGCTAGC |
Internal bar code: | TCACTCGTAGATGAGGGCATGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1014 |
LEAP-Seq percent confirming: | 99.6551 |
LEAP-Seq n confirming: | 11268 |
LEAP-Seq n nonconfirming: | 39 |
LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCAGCTTTGGAGTTGGCTAC |
Suggested primer 2: | ATACCGGGTGTAGTTGCCTG |