| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.040275 |
| Chromosome: | chromosome 17 |
| Location: | 2157398 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g712900 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAATACACACACGCCACTTACCGCTAGC |
| Internal bar code: | TCACTCGTAGATGAGGGCATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1014 |
| LEAP-Seq percent confirming: | 99.6551 |
| LEAP-Seq n confirming: | 11268 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCTTTGGAGTTGGCTAC |
| Suggested primer 2: | ATACCGGGTGTAGTTGCCTG |