| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.040280 |
| Chromosome: | chromosome 12 |
| Location: | 9667061 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554953 | ETF2,ETFB1 | Electron transfer flavoprotein beta subunit; (1 of 1) K03521 - electron transfer flavoprotein beta subunit (fixA, etfB) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGGACAGGTCAGCAGAGCGCAGCGGACT |
| Internal bar code: | TAACTTACTAATACCACTATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 213 |
| LEAP-Seq percent confirming: | 98.3146 |
| LEAP-Seq n confirming: | 175 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGCTGAATTGGCTTTAGG |
| Suggested primer 2: | AGAACTCGCAACCAGCTCAG |