| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.040281 |
| Chromosome: | chromosome 1 |
| Location: | 596413 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003475 | (1 of 2) 3.4.24.35 - Gelatinase B / Type V collagenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGCCTAGGTGCCGTGGTTGCATCGTGT |
| Internal bar code: | ACGCCGCTCCAAGTCGCATCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 628 |
| LEAP-Seq percent confirming: | 99.2924 |
| LEAP-Seq n confirming: | 421 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACACATCCAGTGATCCAG |
| Suggested primer 2: | ATGTATTGGCACTGCAACCA |