Insertion junction: LMJ.RY0402.040305_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.040305
Chromosome:chromosome 11
Location:3452031
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g481093 (1 of 239) IPR016024 - Armadillo-type fold 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATGTGGTGTGATGCCTTCTTTGGCTGCAG
Internal bar code:TCGAGTGTGTTCACCCGTGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:617
LEAP-Seq percent confirming:99.5354
LEAP-Seq n confirming:857
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ATGAGACACACACAACCGGA
Suggested primer 2:CGCTTCTGCTTCTTGCTCTT