| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.040331 |
| Chromosome: | chromosome 13 |
| Location: | 1449184 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572200 | (1 of 1) K03834 - tyrosine-specific transport protein (tyrP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGGATCCGAGGATGACGCCGTCCCACAG |
| Internal bar code: | ACAAGCGCTGATGTTTGATTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 99.6239 |
| LEAP-Seq n confirming: | 8476 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGTACTGTCAGCTTCACG |
| Suggested primer 2: | TTGGTTGTAGGTTGTGGGGT |