| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.040365 |
| Chromosome: | chromosome 1 |
| Location: | 910267 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004850 | TXE1 | Transcription elongation factor; (1 of 1) PTHR30195//PTHR30195:SF13 - TYPE I SITE-SPECIFIC DEOXYRIBONUCLEASE PROTEIN SUBUNIT M AND R // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTATGCGTGGGTCGGAGTTGAGGTGGAGG |
| Internal bar code: | CGCTCGTGAGGAACCATGCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 75 |
| LEAP-Seq percent confirming: | 88.9447 |
| LEAP-Seq n confirming: | 177 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCCTATTGGCTAACGTGG |
| Suggested primer 2: | CATACACACACCAGCCCTTG |