| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.040386 |
| Chromosome: | chromosome 3 |
| Location: | 2049444 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g156400 | SMM8 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 1) 2.1.1.145 - Trans-aconitate 3-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTCACATGTCAGGAGGTGGAGTCAGGTG |
| Internal bar code: | GTTACCGATGTGTCTCCGTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 478 |
| LEAP-Seq percent confirming: | 97.5585 |
| LEAP-Seq n confirming: | 3796 |
| LEAP-Seq n nonconfirming: | 95 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGGAAGGGGTTGCACAAA |
| Suggested primer 2: | TCAGATTCAGTGCGGAACAG |