Insertion junction: LMJ.RY0402.040396_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.040396
Chromosome:chromosome 3
Location:4890378
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g179450 SRR7 (1 of 1) K09624 - protease, serine, 12 (neurotrypsin, motopsin) [EC:3.4.21.-] (PRSS12); Scavenger receptor cysteine rich (SRCR) protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATGGGCCCGGTGCCCTGCCCAAACGCCGC
Internal bar code:CAGTATGATCTTATGTGCCGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:465
LEAP-Seq percent confirming:32.3077
LEAP-Seq n confirming:42
LEAP-Seq n nonconfirming:88
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTAAATGTGTAGCCAGCGCA
Suggested primer 2:AACTCGCAGGCTGTAAAGGA