| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.040406 |
| Chromosome: | chromosome 12 |
| Location: | 1756492 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512450 | (1 of 12) PF04749 - PLAC8 family (PLAC8) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGGAATGCAGGCATTACGTATCGGCGTA |
| Internal bar code: | TCTGGGCTAGTTTCCTCCAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1024 |
| LEAP-Seq percent confirming: | 99.5629 |
| LEAP-Seq n confirming: | 5694 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATAACTTGCCAACGTTCCG |
| Suggested primer 2: | AGTCTTACATGTCGGGTGGC |