| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.040417 |
| Chromosome: | chromosome 16 |
| Location: | 685161 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g691500 | (1 of 1) K18470 - Rho GTPase-activating protein 1 (ARHGAP1, CDC42GAP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCCTGCAATCACCGCCCCATTCACTTC |
| Internal bar code: | ACCGAGGCGATTCGCGTCTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 972 |
| LEAP-Seq percent confirming: | 99.112 |
| LEAP-Seq n confirming: | 4018 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACACGGTACACAAGGTCG |
| Suggested primer 2: | GCTTCCTGGCTGTTGAGTTC |