| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.040440 |
| Chromosome: | chromosome 3 |
| Location: | 7624073 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204400 | (1 of 1) K09651 - rhomboid domain-containing protein 1 [EC:3.4.21.-] (RHBDD1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGCTCCGCCCCCGCCCTTGCCCCCGCC |
| Internal bar code: | TTCGTAGGCTCGCAACAACGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 213 |
| LEAP-Seq percent confirming: | 97.9798 |
| LEAP-Seq n confirming: | 194 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGTATTCAGAGGTGGAGA |
| Suggested primer 2: | CTGACTTTGTGTGGTGGTGG |