Insertion junction: LMJ.RY0402.040448_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.040448
Chromosome:chromosome_6
Location:3925835
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre06.g278186 antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):CTGTGCACAAACAGGTGCTTACTAGTTAGC
Internal bar code:CAACTCGCCGGCACTCTCATGT

Confirmation - LEAP-Seq

LEAP-Seq distance:416
LEAP-Seq percent confirming:96.8309
LEAP-Seq n confirming:3361
LEAP-Seq n nonconfirming:110
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTGTAATTGTTTGCGCTC
Suggested primer 2:GACAGTCTGATTGACGCGAA