| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.040451 |
| Chromosome: | chromosome 17 |
| Location: | 2192686 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g713150 | FAS8,FLA3 | FAS1 domain containing protein;; (1 of 21) PF02469 - Fasciclin domain (Fasciclin) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTGGTCAGCGTGGTACCCTTGGCCTTGA |
| Internal bar code: | AGACGGGGACTTGCCTACGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 894 |
| LEAP-Seq percent confirming: | 99.6469 |
| LEAP-Seq n confirming: | 5079 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTCAGATCAGGTCAGCA |
| Suggested primer 2: | CAGCACTGGCAGGACTATCA |