Insertion junction: LMJ.RY0402.040478_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.040478
Chromosome:chromosome 6
Location:4920792
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g279450 MUT68 (1 of 7) PTHR11584//PTHR11584:SF381 - SERINE/THREONINE PROTEIN KINASE // PROTEIN PQN-80, ISOFORM B; protein kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCATCGATGCCTTCCCTGACCTTGCCCTT
Internal bar code:GGACGCGACGACGGAGCGCGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:466
LEAP-Seq percent confirming:96.5259
LEAP-Seq n confirming:13003
LEAP-Seq n nonconfirming:468
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTGGTTCGCTGACATGC
Suggested primer 2:GTCTGGGTAGCTCATGCGAG